Abstract
The adenosine-A3 receptor is one of four known G-protein coupled receptors activated by the nucleoside adenosine. Here we use fluorescence correlation spectroscopy (FCS) in conjunction with pharmacological and molecular biology approaches to investigate the diffusion characteristics of different activity states of the human A3-receptor. Initial FCS experiments using Chinese hamster ovary (CHO) cells expressing the wild type human A3-receptor and the fluorescent adenosine receptor antagonist XAC-X-BY630 revealed both fast and slow moving complexes at the cell membrane, with average diffusion co-efficients of 1.58±0.16 μm2/s (τD2) and 0.081±0.007 μm2/s (τD3), respectively.
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