Studying the cellulolytic activity of microorganisms isolated from stained painted walls with reference to certain factors: a cross sectional study

Abstract

Investigations of enzymatic activities amongst microbial species on stained painted walls are not common especially in the tropics. Meanwhile, an organism’s enzymatic activity is a key index of its biodegradation potential. We conducted a cross-sectional study to assess the cellulolytic profiles of microorganisms isolated from stained painted buildings. A total of eight microorganisms comprising one bacterium [Pseudomonas aeruginosa CH01 (KY511067.1)] and seven fungi [Meyerozyma guillermondii MB14B1 (LT615287.1), Meyerozyma caribbica CBS:5674 (KY104219.1), Aspergillus aculeatus A1.9 (EU833205.1), Aspergillus sp. SL2 (KC178662.1), Fusarium proliferatum2705 (EU272509.1), Cerrena sp. N10CC2a (FJ010208.1) and Candida tropicalis UZ31_13 (KM361510.1)] were utilized for the study. The results showed that all isolates possessed exoglucanase and endoglucanase activities ranging from 0.174 to 1.554 IU/ml and 0.062 to 2.120 IU/ml, respectively. Analysis of the interplay of organism cellulolytic activity at various environmental conditions showed that endoglucanase activity was optimal in Cerrena sp., while the highest exoglucanase activity was recorded in P. aeruginosa.