Studying Some Novel Biochemical and Immunological Markers in a Sample of Iraqi Women with Polycystic Ovarian Syndrome

Abstract

Polycystic ovarian syndrome (PCOS) considers as the most common disorder among women during reproductive age. Till now, the pathogenesis of PCOS stay unknown, and there is evidence considered PCOS as a low-grade inflammatory disease. The present study was designed to find out relationship of new biochemical markers, like fetuin-A, and immunological markers, like sirtuin-1, auto-antibodies (anti-histone antibody (AHA),anti-ovarian antibody (AOA)), and interleukins (IL-17, IL-23) with PCOS in studied patients when compared with healthy control group. Also, the study aimed to find the possible relationship between PCOS and autoimmunity.A total of 60 PCOS women and 30 healthy women, matching in average age and body mass index (BMI), were enrolled in this study. Women with PCOS were attend to AL -Nahrain University High Institute for Infertility Assisted Reproductive Technology, in Baghdad between Septembers to December/2018. Blood samples were aspirated from both groups to study the biochemical and immunological markers byusing enzyme linked immunosorbent assay (ELISA). The obtained results showed highly significant elevation (P<0.01) in luteinizing hormone (LH) serum levels of patients group. Regarding the results of serum fetuin-A, sirtuin-1, auto-antibodies, and interleukins levels, highly significant differences were noticed between patients and control group (P<0.01). On the other hand, the LH recorded a direct correlation at highly significant level with sirtuin-1 and auto-antibodies (AH, AOA). Also LH showed a direct correlation at significant level with ILs (17, 23). Sirtuin-1 showed a direct correlation at highly significant level with auto-antibodies (AHA, AOA) and ILs (17, 23). Both auto-antibodies have direct correlation at highly significant level with ILs (17, 23) and with each other, in addition to their correlation with sirtuin-1, auto-antibodies (AHA, AOA) and LH. The current study showed that the studied markers were elevated significantly in patients group when compared with control. These findings confirm the possibility that PCOS may attributed to an autoimmune disease.