Studying safe storage time of orange peel (Citrus reticulata) using high-throughput sequencing and conventional pure culture.

Fu Wang,Fengqing Li,Youping Liu,Xin Zhang,Sujuan Liu,Hongping Chen,Lin Chen

doi:10.1002/fsn3.866

Abstract

Aspergillus is a fungal genus widely studied all over the world because some species are known allergens and opportunistic human pathogens. The dynamic growth of Aspergillus is a prerequisite for establishing safe storage time of orange peel. In this paper, high‐throughput sequencing technique was used for the first time to analyze the diversity and structure of fungi in the same batch of samples at different periods of time, and 20 batches of fresh orange peel and 56 batches of dried peel were verified. Results shown that the orange peel gradually began to grow Aspergillus fungal after storing for 240 days, and the abundance became maximum at 270 days and then decreased. These results suggest the safe storage time should be from January to August. And orange peel should be dried in August to prevent rapid propagation or metabolic toxicity production of Aspergillus fungi.

Highlights

Citrus reticulata Blanco is widely cultivated in the world
The orange peel can be processed as food, tea drinks, seasoning, or even herb-medicine (Rachma, Huong, Ria, Claes, & Mohammad, 2014; Zheng, Zeng, Peng, Wu, & Su, 2018), but it is easy to mildew during storage and infect pathogenic fungi or toxins, endangering the health of consumers
The index of coverage refers to the sequence coverage of each sample, and the higher the value of coverage, the greater the probability of sequence detection in the sample, and the smaller the probability of not being detected, which directly represents the authenticity of the sequencing results of the sample (Kraková, Šoltys, Budiš, Pangallo, & Szemes, 2016)

Summary

Introduction

Citrus reticulata Blanco is widely cultivated in the world. The orange peel can be processed as food, tea drinks, seasoning, or even herb-medicine (Rachma, Huong, Ria, Claes, & Mohammad, 2014; Zheng, Zeng, Peng, Wu, & Su, 2018), but it is easy to mildew during storage and infect pathogenic fungi or toxins, endangering the health of consumers. The microbial community structure, including composition, abundance, and diversity, is not fully understood during storage of orange peel, which we cannot put forward the safe storage time and scientific safe storage measures for orange peel. Pure culture methods have been used to identify pathogenic fungi. In these methods, isolation, identification, and biological characteristics of pathogenic fungi were studied (Yan, Chen, & Deng, 2007; Yang et al, 2015). Isolation, identification, and biological characteristics of pathogenic fungi were studied (Yan, Chen, & Deng, 2007; Yang et al, 2015) These conventional methods are limited in identifying fungal communities in full scale.

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Conclusion