Abstract
This article presents the method of buck genome stability evaluation based on micronucleus assay. This method was used in rabbits breeding for the first time. It allows to find opportunities for accelerated creation of White Giant rabbits population according to their reproductive ability and genomic stability. The micronucleus test was performed by microscopic examination of blood films (blood: normal saline – 1: 1, respectively). Before staining blood films were fixed in methyl alcohol. The dye is a ready-made solution of Romanovsky-Giemsa. The preparations were examined according to the frequency of erythrocytes occurrence with microkernel (EMN) under a Micros Austria pD 2385 microscope (magnification 1000 times). Microkernel were counted in 3000 erythrocytes and expressed in ppm (‰). Analysis of the White Giant bucks’ cytogenetic characteristics showed that the frequency of red blood cells with micronuclei occurrence ranged from 0.2-1.8%, with an average of 0.88 ± 0.09%.These indicators fell within the normal range for the bucks used in reproduction (not exceeding 2 ‰ for farm animals).
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