Studying of Oxidative Stress and Activity of Some Enzymes in Cerebrospinal Fluid from Children with Hydrocephalus Disease

Abstract

This research include studying the oxidative stress state and the activity of some enzymes in the cerebrospinal fluid (CSF) from children with hydrocephalus by the determination of (15) Biochemical parameters which include: Acetylcholinesterase (AChE), Pseudocholinesterase (PChE), Monoamin oxidase A (MAOA), Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Alkaline phosphatase (ALP), Creatinine kinase (CK), Lactate dehydrogenase (LDH), Superoxide dismutase (SOD), Uric acid (UA), Vitamin D, Total protein (Tp), Albumin (Alb), Ceruloplasmin (Cp), Malonadialdehyde (MDA), The study was done on (176) child with hydrocephalus (110) were male , (66) female and (67) healthy child as control group (39) were male and (28) female ,the age of children with hydrocephalus and control group ranged from 1-14 month for both sexes. The results showed that there was a significant increase in the activity of AChE, PChE, MAOA, AST, ALT, ALP, CK and LDH in CSF for children with hydrocephalus compared with healthy group for both sexes, these result were indicated that these parameters may be good biochemical markers for this disease . Beside of that, the results showed there was a highly oxidative stress in the patients for both sexes, so there was a significant increase in the activity of SOD, Tp, Alb and oxidant indicator of MDA, with a significant decrease in vit. D, uric acid and Cp levels in CSF patients compared with healthy group for both sexes,