Studying genotype of Kishmish No. 342 and manifestation of parthenocarpy under conditions of the Anapa ampelographic collection

Abstract

Тhe genotype of Kishmish No. 342 (Hungarian Kishmish), along with the manifestation of parthenocarpy in this variety, was studied. Тhe research included plants of the Kishmish grape variety No. 342 growing in the Anapa ampelographic collection. Тhe evaluation of the parthenocarpy category was carried out for three years; the results were compared with the data on weather conditions. Тhe mass of vestigial structures was determined by the extraction of the ovules from berries and their gradual dehydration in a drying cabinet until constant weight. DNA extraction was carried out using a CTAB buffer. Genetic studies were performed by polymerase chain reaction (PCR). DNA profiling was performed using SSR markers (VVS2, VVMD5, VVMD7, VVMD27, VVMD25, VVMD32, VrZAG62, VrZAG79) standard for DNA certification of grape varieties. Closely coupled microsatellite markers, including UDV305, UDV737 (Rpv3) and UDV343, UDV360 (Rpv12), SC8-0071-014, SC47-18 (Ren1), ScORGF15, were used to identify the allele status of gene loci resistance to mildew (Rpv3, Rpv12) and powdery mildew (Ren1, Ren3, Ren9).02, GF15-42 (Ren3) and CenGen6 (Ren9), recommended for such work. Тhe results of PCR analysis were evaluated by capillary electrophoresis using a Nanophor-05 sequencer. Тhe research results show the variation in the parthenocarpy classes from 2 to 4 under the weather and climate conditions of Anapa in 2019–2021; the largest mass of vestigial structures was observed for the year characterised by the hottest flowering conditions and the greatest heat summation prior to the flowering period. A DNA certificate of the Kishmish variety No. 342 was established (VVS2133 143 VVMD5236 238 VVMD7251 253 VVMD27182 182 VrZAG62188 194 VrZAG79247 255 VVMD25241 255 VVMD32240 272); the origin of the genotype (Villard blanc x Perlette) was confirmed according to DNA analysis. On the basis of the DNA marker analysis, the presence of gene loci resistant to mildew (Rpv3) and powdery mildew (Ren3 and Ren9) in the genotype of Kishmish No. 342 was shown.