Abstract
High—speed atomic force microscopy has proven to be a valuable tool for the study of biomolecular systems at the nanoscale. Expanding its application to larger biological specimens such as membranes or cells has, however, proven difficult, often requiring fundamental changes in the AFM instrument. Here we show a way to utilize conventional AFM instrumentation with minor alterations to perform high-speed AFM imaging with a large scan range. Using a two—actuator design with adapted control systems, a 130 × 130 × 5 μm scanner with nearly 100 kHz open—loop small-signal Z—bandwidth is implemented. This allows for high-speed imaging of biologically relevant samples as well as high-speed measurements of nanomechanical surface properties. We demonstrate the system performance by real-time imaging of the effect of charged polymer nanoparticles on the integrity of lipid membranes at high imaging speeds and peak force tapping measurements at 32 kHz peak force rate.
Highlights
High—speed atomic force microscopy has proven to be a valuable tool for the study of biomolecular systems at the nanoscale
In order to make high-speed AFM (HS-AFM) available to the broad nanotechnology community it is essential to enable large scan ranges in all directions at high speeds[15,16,17]
At room temperature, the dilauroyl-sn-glycero-3-p hosphocholine (DLPC) is in the fluid phase and the DPPC is in the gel phase
Summary
High—speed atomic force microscopy has proven to be a valuable tool for the study of biomolecular systems at the nanoscale. Current HS-AFMs are highly specialized, complex instruments tailored to measuring the dynamics of nanoscale systems such as molecular motors[6], membrane proteins[7,8], or antibodies[9] with temporal imaging rates of less than one second per image. These measurements have stirred high hopes for advancements in other bio- and nanotechnology fields as well. The length extensional resonance (Z-ringing) is visible as horizontal ripples that follow sharp steps in the topography
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
