Study the Role of HLA-DQB1 Alleles and Interlukine-17 in Women with Rheumatoid Arthritis in Al Najaf Governorate

Abstract

Objectives: The study aim to detect the genetic relationship between HLA-DQB1 alleles with Rheumatoid Arthritis and measurement level of Interleukin 17 and it is relationship with immune antibody that related with Rheumatoid arthritis.Methodology: this study included 60 women have Rheumatoid arthritis whom visited Al-Sadder Medical City in Al Najaf Governorate from February 2015 to July 2015 , and confirmed the diagnosis by use specific antibody test related with disease include (Anti-CCP) Anti Cyclic Citrullinated Peptide, (RF) Rheumatoid Factor. This study included 60 women as healthy control group, and HLA-DQB1allele has been identified for both group by MR. Spot (SSO System). Interleukin 17 was detected in serum for both group by using ELISA Technique, this study designated as case control study and the results was analyzed by using statistical methods (SPSS version 20,t-test groups, Chi square test, persons correlation -r)Results: the result of this study showed that frequency of HLA-DQB1*6 in patients group more frequent about 73.3% and showed (OR=3.85) that mean this allele represent as risk factor for women whose carried it that mean more susceptible for infected with Rheumatoid arthritis about 3.85 times, while in control group showed HLA-DQB1*3 more frequent (66.6%) that may as a protective factor from disease. This study showed increasing in level of Interleukin 17 in serum of women have Rheumatoid arthritis and that registered high significant differences (P<0.001) when compare with level in serum of control group. Moreover there is significant correlation between presence of (anti-CCP) Anti Cyclic Citrullinated Peptide and RF(Rheumatoid Factor) antibodies with increasing in level of Interleukin 17 in patient group.Conclusions: this study concluded that important of detection HLA-DQB1*6 as a risk factor for disease and possible to prognostic for inducing disease for women have that allele. Using of Interleukin 17 test with Rheumatoid arthritis patients that may due to important role in progressive of disease and associated it with producing antibodies from Rheumatoid arthritis.