Study the Potential Nephro-protective Effect of Stem Cells compared to Perindopril on Experimentally Induced Nephropathy

Abstract

AKI is as a public health problem worldwide.Gentamicin is one of the leading causes of drug-induced nephrotoxicity.Objective:To assess the reno protective effect of ACEI Perindopril and BMDMCs on gentamicin induced nephrotoxicity in rats.The animals were divided into four groups:group A (Control),GroupB(Gentamicin-treated):It consists of 40 rats received sub-cutaneausly Gentamicin in a dose of 80mg/kg once daily for 7 consecutive days and subdivided into 4 subgroups:B-I:10 rats as acontrol group.B-II:consists of 10 rats on the eighth day after Gentamicin injection they received oral Perindopril at doses of 6 mg/kg per day for4weeks.B-III:consists of10rats on the eighth day after Gentamicin injection, stem cells (MSC) were injected in tail vein at dose of 5x106 cells/rat. B-IV:consists of 10 rats on the eighth day after Gentamicin injection, stem cells (MSC) were injected in tail vein and oral Perindopril. Results: Gentamicin induced kidney damage in rats was indicated by significant increase in the levels of serum urea, creatinine, potassium and tissue Malondialdehyde (MDA)concomitant with significant decrease in levels of serum sodium and tissue Super oxide dismutase (SOD)as compared to normal control group. Treatment with Perindopril significantly decreased serum urea and creatinine and significantly decreased tissueMDA.Treatment with BMSCs significantly decreased serum urea and creatinine, significantly increased serum sodium and decreased serum potassium and significantly decreased tissue MDA and increased tissue SOD.These data indicate that treatment with stem cell in AKI can give better results than Perindopril and should encourage clinical studies to evaluate the potential benefit of MSC administration,because their infusion in humans is feasible and safe.