Study the Physico-Morphologic Parameters of Neat Cryopreserved Murrah Semen

Abstract

Artificial insemination with frozen – thawed spermatozoa was introduced in most of the developing countries more than three decades ago, yet it has not been successfully applied in large scale. More than 50% spermatozoa are usually injured during the cryopreservation process. Injuries caused by cryopreservation are more likely due to increasing solute concentration and the formation of intracellular and extracellular ice crystals during cryopreservation [54] leading to significant decline in semen quality and alterations in sperm morphometrix. Therefore, the present study was designed to study cryopreservation of buffalo semen and the effect of semen cryopreservation on determinant like semen fertility viz. physico-morphologic. Forty eight semen samples collected from eight Murrah buffalo bulls maintained at Central semen station, Bhopal were included in the study for the study of physico-morphological characters of neat and cryopreserved semen. The overall average of the eight bulls for neat semen volume mass activity, progressive motility, live %, sperm concentration, head, midpiece, tail, and total sperm abnormalities was 2.89±0.14, 3.64±0.07, 71.56±0.37, 86.22±0.66, 963.92±38.51, 5.58±0.28, 2.18±0.32, 2.09±0.21 and 9.85±0.23 %, respectively. There was significant (P<0.05) between bulls and ejaculate variation in semen volume, sperm concentration, sperm midpiece and tail abnormalities. No significant differences between bulls as, however, recorded in sperm mass activity, progressive sperm motility, live sperm percent, sperm head and total sperm abnormalities.