Study the Phenotypic and Antimicrobial Susceptibility of Pseudomonas aeruginosa Isolated Clinically from Baghdad Hospitals

Abstract

Pseudomonas aeruginosa, is negative to gram stain, take the rod shape and considered as strictly aerobic. the most affect bacteria in nosocomial infection. P.aeruginosa is an opportunistic pathogen bacterium that can made serious infections in human who are immune compromised, such as urinary tract infection, skin and ear infection, and others. One hundred twenty one sample were taken from patients in different sites of infections (ear, wound, UTI , burn) from AL-Yarmouk, Ghazi Al-Hariri hospitals in Baghdad during the period from November 2016 to February 2017. Fifty seven isolates were diagnoses as P. aeruginosa by characteristic in culture media, tests and API 20E. forty isolates involved in our study ten from each source. Antibiotic susceptibility test performed for forty clinically isolates of P. aeruginosa by the disk diffusion method against some antibiotics belong to different group and the results revealed that bacteria is multi-drug resistance (MRD) as well, revealed the most compound that have activity against Pseudomonas. aeruginosa were Imipenem, Pipracillin, and Ceftazidime. Biofilms are currently estimated and the P. aeruginosa reflect high ability to produce biofilm. All isolates used in this study formed biofilm with differences in thickness of formed layer. In this study, we concluded P. aeruginosa is one of the most common gram-negative bacteria involved in hospital infections causing opportunistic infection because they have intrinsically and acquired resistant to a number of antimicrobial agents and produces a number of exoproducts which are implicated in the pathogenesis of P. aeruginosa infections.