Abstract
Artificial insemination with frozen – thawed spermatozoa was introduced in most of the developing countries more than three decades ago, yet it has not been successfully applied in large scale (Anzar et al., 2003). More than 50% spermatozoa are usually injured by the cryopreservation process (Watson, 1995) injuries due to cryopreservation and more likely due to the increased solute concentration and the formation of intracellular and extracellular ice crystals during cryopreservation (Mazur, 1984) leading to a significant decline in semen quality and alterations in sperm morphometrix. Therefore, the present study was designed to study cryopreservation of buffalo semen and the effect of semen cryopreservation on determinant like semen fertility viz. sperm morphometry. Forty-eight semen samples collected from eight Murrah buffalo bulls maintained at Central semen station, Bhopal were included for the study of physic-morphological characters of neat and cryopreserved semen. Various measurements of the sperm head (length, width, base, ellipticity, elongation, head shape and area) and sperm tail (length of Midpiece and length of tail) were recorded in neat and cryopreserved semen. The average sperm measurements recorded were 7.24 ± 0.06, 4.26 ± 0.05, 2.65 ± 0.79, 11.92 ± 1.53, 43.12 ± 2.79, 61.97 ± 0.50, 25 ± 0.07, 19.13 ± 2.58, 0.59 ± 0.01, and 59.07 ± 0.94, respectively. There was a significant (P<0.05) decline of sperm head length, head width, midpiece length, tail length, total length and head area during the process of cryopreservation. However, no significant difference between width at base, ellipticity, head shape and elongation of the spermatozoa in neat and cryopreserved semen was recorded.
Highlights
Buffalo has been an important animal species of Indian subcontinent since prehistoric time
(P
The highly significant positive correlation of total sperm length with head length, mid piece length and tail length recorded in the present study suggest that sperm with longer heads are associated with greater overall length
Summary
Buffalo has been an important animal species of Indian subcontinent since prehistoric time It contributes substantially in terms of milk, meat and hide to the national economy. Minimal changes in protocols for processing and freezing bull semen have been implemented in the past 20 years. This limited rate of progress is due to lack of complete understanding of the factors and interactions that alter spermatozoa viability upon processing, freezing and thawing. Considering the importance of minimizing damage to the spermatozoa during cryopreservation process and need of accurate assessment of the sperm fertility in implementation of any artificial insemination programme, the present study was conducted with the following objective to study the morphometrix of fresh and cryopreserved Murrah bull spermatozoa
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
