Abstract

This study was conducted to investigate the effect of toothpastes used for oral health by acting as antimicrobial against biofilm formations. Different types of toothpastes were selected, including those were made of natural materials and others with artificial chemicals using herbal additives. Five tooth pastes (Lacalut®, Paradontax®, Colgate®, Miswak®, Sanino®) were used on microbiota spectrum of spp. Bacteria (s. mutans, s. viridans) obtained by swab plaque accumulated in teeth from different patients over a period of 2021, the exclusion criteria from the investigation were patients receiving concurrent antibiotic treatment for any purpose. This research was conduct in central environmental laboratory at Baghdad University. Each toothpaste is diluted with distilled water to prepare 0.2 and 0.3 concentrations. The estimation of bacterial spectrum was performed in qualitative manner using standard methods for microbiological inoculation tissue plate culture (TPC)were performed (for bacterial presence) and quantitative qualification of the subjects by, Elisa instrument. It was found that toothpaste which contains natural materials is particularly effective in improving oral health and reducing the incidence of gingivitis caused by microbial bacterial presence. This revealed that the naturally occurrence of different groups of organic compounds flavonoids and phenolic acids (esters), are responsible for many of the biological activities against oral bacteria.

Highlights

  • Biofilm formation is a natural scheme with inside the oral environment, but needs to be controlled through normal brushing that permits to supply from progress of caries and periodontal infections (1)

  • Bacteria (s. mutans, s. viridans) obtained by swab plaque accumulated in teeth from different patients over a period of 2021, the exclusion criteria from the investigation were patients receiving concurrent antibiotic treatment for any purpose

  • To their anti-caries impact, maximum toothpastes may be widely categorized as having cosmetic with healthy oral cavity (4) Orally infection by microbial bacteria leads to formation dental plaque which means adhering the bacteria to the soft tissue and aggregate biofilm surrounding the effective area that leads to plague of gingival,the crevice fluid supply all nutrients needed for the continuous rising bacterial growth and infected place that became difficult to treated by antibiotics because the drug used cannot penetrate and achieve the infective site

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Summary

Introduction

Biofilm formation is a natural scheme with inside the oral environment, but needs to be controlled through normal brushing that permits to supply from progress of caries and periodontal infections (1). Toothpastes enhance oral hygiene with the aid of improving the impact of mechanical scrubbing with a toothbrush and handling over medicinal agent to the oral cavity To their anti-caries impact, maximum toothpastes may be widely categorized as having cosmetic with healthy oral cavity (4) Orally infection by microbial bacteria leads to formation dental plaque which means adhering the bacteria to the soft tissue and aggregate biofilm surrounding the effective area that leads to plague of gingival ,the crevice fluid supply all nutrients needed for the continuous rising bacterial growth and infected place that became difficult to treated by antibiotics because the drug used cannot penetrate and achieve the infective site. The consequence cleaning and enhancing agent is to remove any adherent layer on the tooth from backward to forward to reduce the pain perseverance of giving foam to crash and get rid of the debris, Flavors are possibly the most essential part of toothpaste as of consumer preferences (6) This observation developed out to study the impact of toothpastes used for oral qualification as overall antimicrobial performance in the direction of biofilm formations

Materials and Methods
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