Abstract
The present study was designed to investigate the role of P. odoratissimum aquatic extracts in reducing the genotoxic effects of metronidazole in mice in vivo and human blood lymphocyte in vitro. The parametrers which evaluated in mice were used: mitotic index and chromosomal aberrations in bone marrow, while for human blood lymphocyte were mitotic index, blast index, replicative index, sister chromatid exchange and chromosomal aberrations. The cytogenetic effects of the drug and plant aquatic extracts were investigated after four days of oral administration for mice with metronidazole and aqueous extract at doses 400mg/kg and 100 mg/kg respectively while the concentrations of metronidazole and aqueous extract in human blood lymphocyte culture was 80µg/ml, and 10µg/ml respectively. An interaction study of plant extract with metronidazole was carried out through three types of treatments (before, after and mixture of plant extract and drug treatment) to determine the activity of P. odoratissimum aqueous extract in reducing the side effects of drug both in vitro and in vivo. Aquatic extract of P.odoratissimum at the concentration of 10µg/ml, showed a protective value against the genotoxic effect of metronidazole at 80µg/ml. concentration .In mouse bone marrow cells and human blood lymphocyte culture, this was more pronounced in pre-treatment and simultaneous treatment than in post-treatment. So P. odoratissimum aquatic extract is considered as desmutagen in the first order and bioantimutagen in the second order, as a result for its ability to repair CA and increase MI in mouse system and in human blood lymphocyte culture system . It also had the ability to increase BI and RI and decrease SCE in human blood lymphocytes culture in vitro.
Highlights
Herbal medicines include dietary supplements that contain herbs, either singly or in mixtures which called botanicals, herbs are plants or plant parts used for their therapeutic properties
Metronidazole is indicated in the treatment of bone, joint and brain abscess infections caused by Bacteroides species, including the B. fragilis group (B. fragilis, B. distasonis, B. ovatus, B. thetaiotaomicron, B. vulgatus) [4]
Another important infections treated by MTZ is that of the central nervous system (CNS) infections including meningitis and in the treatment of endocarditis caused by Bacteroides species, including the B. fragilis group [5]
Summary
Herbal medicines include dietary supplements that contain herbs, either singly or in mixtures which called botanicals, herbs are plants or plant parts used for their therapeutic properties. Concentration .In mouse bone marrow cells and human blood lymphocyte culture, this was more pronounced in pre-treatment and simultaneous treatment than in post-treatment. P. odoratissimum aquatic extract is considered as desmutagen in the first order and bioantimutagen in the second order, as a result for its ability to repair CA and increase MI in mouse system and in human blood lymphocyte culture system .
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