Study the Effect of Ferula hermonis Extract on HepG2 cell line culture

Abstract

Ferula hermonis Boiss (Apiaceae) is known in the Midleast as
 "zallouh". In the last decade, scientists paid attention to study
 
 chemistry and biological activity of the plant. In this study Ferula
 hermonis were collected and evaluated for cytotoxic activity. The
 plant was extracted with methanol The cytotoxic effect of the
 extracts was examined in-vitro on human cell line (Human liver
 carcinoma cell line HepG2). Results were shown that significant
 decrease of HepG2 cells viability. The effect of F. hermonis on cell
 viability of HepG2 cell line culture show that plant extract cause
 significant decrease ( p< 0.05) of HepG2 cell viability, and this
 decrease in cell viability is increased with the increasing the extract
 concentrations in comparison with the negative control, and the
 growth inhibition percentage reach to (78.3%).
 Introduction
 
 Ferula hermonis Boiss. (Apiaceae), commonly known as " Shilsh-
 el-zallouh" , "Hashishat-al-kattira" or "The Lebanese Viagra" ,is a
 small perennial shrub that grows abundantly at more than 2500 m on
 the high mountain areas of northern Lebanon, and on the biblical
 Mount Hermon in Southern Lebanon, (El-Taher et al., 2001). Middle
 East herbalists have used the seeds and roots of this plant for
 centuries as a folk remedy as an aphrodisiac to treat frigidity in
 women, and erectile and sexual dysfunction in men by increasing
 blood flow to sexual organs. The antimicrobial activity of the crude
 extract and the isolated compounds has been reported (Hilan et al.,
 2007). Previous phytochemical studies on this plant have revealed
 the presence of various sesquiterpenes, mainly of the daucane ester
 type (Galal et al., 2001). On roots of Ferula hermonis, several
 sesquiterpenes have been isolated, most of which display biological
 activities. The active constituents are believed to be daucane alcohols