Study the effect of Ethanolic extract of Anethum graveolens L. on Aspirin induced Gastric Ulcer in Male Guinea Pigs.

Abstract

The objective of the present study is to investigate the effect of ethanolic extract of Anethum graveolens L. on Aspirin – induced gastric ulcers in guinea pigs. thirty guinea pigs divided in to five groups (6 each group), first group received oral administration 2ml of normal saline served as negative control. 500mg/kg B.W of Aspirin was used to induced ulcer in guinea pigs for 3 days. after checking for presence of ulcers, the ulcerogenic guinea pigs were divided in to four groups, 2 groups were treated with ethanolic extract of Anethum graveolens L. at doses of (150mg/kg, 300mg/kg) respectively, while a group received cimetidine at dose 20mg/kg for 10days and the last group was the ulcer untreated control (control positive). At the end of the study the animals were sacrificed, the stomach was prepared for estimating, gastric PH , gastric ulcer index, total gastric area, gastric fluid volume, serum MDA and tissue MDA, in addition a gross and histological examination of the stomach. There were significant decrease in PH in control positive , while it showed significant increase(P= 0.05) of gastric ulcer index, total gastric area, gastric fluid volume, serum MDA and tissue MDA compared with the negative control group. The study also revealed significant decrease (P=0.O5) in gastric ulcer index, gastric volume, total gastric area, serum MDA and tissue MDA with a significant increase(P=0.05) in gastric PH in groups treated with ethanolic extract and in the group treated with cimetidine compared with the positive control group. Histological studies showed many pathological changes in stomach ?? control positive group but in the treated with ethanolic extract of Anethum graveolens L at dose 300mg/kg group the histopathological changes near to the normal status, whereas, higher dosage of extract (300mg/kg) was more superior to cimetidine drug in its protection of the stomach