Study the C/T Single Nucleotide Polymorphism at Tyrosine Kinase Domain of Insulin Receptor Gene in Patients with Polycystic Ovary Syndrome in Babylon Province

Abstract

Polycystic ovary syndrome (PCOS) is the commonest gynecological endocrinopathy, is characterizedby chronic anovulation and hyperandrogenism and is also associated with increase insulin secretion andmetabolic disease and insulin resistance. Present study aims to examine the association between the singlenucleotide polymorphism (rs1799817) of exon 17 of the insulin receptor (INSR) gene and polycystic ovarysyndrome (PCOS) in a Babylon women. Fifty women with PCOS and fifty healthy Iraqi women, age andBMI matching were achieved between study groups. Biochemical parameters that estimated were fastingglucose, fasting insulin, luteinizing hormone, testosterone. His 1058 C/T polymorphism at the tyrosinekinase domain in the INSR gene was analyzed by restriction fragment length polymorphism (RFLPPCR). Significant increase in the levels of fasting glucose fasting insulin, HOMA-IR, LH, testosteronewere noticed in patient when compared with control. TT genotype frequency was higher in PCOS patientswhereas CC genotype was higher in control women. All parameters that estimate in this study were higherin TT genotype of patient group. In conclusion there are an association of C/T polymorphism at His1058 ofINSR in patients women with PCOS in Babylon Province. Insulin resistance in PCOS patients might differfrom these that result from obesity.