Abstract
This study was aimed to purification the biosurfactant that produced from Lactobacillus plantarum isolated from clinical samples of iraqi healthy women by column chromatography through silica gel column (3.5 × 30 cm) using solvent system (chloroform : methanol, 2:1) and characterization the purified product by Thin layer chromatography (TLC), Fourier Transform Infrared Red spectroscopy (FTIR) and Gas chromatography (GC) mass then evaluated its antibacterial and anti-adhesive activity. The results shown the higher emulsification activity (E24%= 63) and lower the surface tension to 23 mN/m in synthetic MSM, while the natural media gave (E24% =71) and lower surface tension to 19 mN/m. The using TLC technique indicated presence of lipopeptide in the biosurfactant with Rf value = 0.82. Antibacterial and antiadhesion activities were evaluated against some pathogenic bacteria, including Pseudomonas aureginosa and Staphylococcus aureus. The results showed a higher inhibitory effect of biosurfactant at concentration 200 mg/ml on S. aureus and P. aeruginosa with the inhibition zone 27 mm and 33 mm respectively in BCDFTM media. While purified biosurfactant with concentration 200 mg/ml produced in MSM media had an effect on S. aureus and P. aeruginosa with the inhibition zone 21 mm, and 18 mm respectively. The anti-adhesion activity of purified biosurfactant against p.aeruginosa ranging from (34.70 ± 5.256) to (11.7 ± 9.7) produced from natural media BCDFTM and was higher than the anti-adhesion activity of purified biosurfactant against the same pathogen ranging from (46.95 ± 7.37) to (20.15 ± 2.805) produced in MSM.
Highlights
Biosurfactants is amphipathic molecules and mainly excretions by micro-organisms outside the cells, and sometimes attached to the cells, mostly during growth on water immiscible substrates
BS are released by probiotic lactic acid bacteria (LAB), including Lactobacillus helveticus (26), Lactobacillus plantarum (16), and Lactococcus lactis (20)
The cell walls of gram negative bacteria are usually resistant to lipophilic solutes because they consist of a peptidoglycan layer and an additional outer membrane rather than gram-positive bacteria cell walls, which contain peptidoglycan, which makes gram positive more sensitive (12)
Summary
Biosurfactants is amphipathic molecules and mainly excretions by micro-organisms outside the cells, and sometimes attached to the cells, mostly during growth on water immiscible substrates. BS can potentially be utilized as therapeutic agents because they are safe and have antibacterial, antifungal, and antiviral functions They disturb the membranes which results in an increase of the membrane permeability, followed by cell lysis and loss of metabolites. The cell walls of gram negative bacteria are usually resistant to lipophilic solutes because they consist of a peptidoglycan layer and an additional outer membrane (narrow outer wall) rather than gram-positive bacteria cell walls, which contain peptidoglycan (loose outer wall), which makes gram positive more sensitive (12) This may be because lipopeptide biosurfactant causes loss or damage of the peptidoglycan layer may inhibit the biochemical reactions in the cell wall and prevent peptidoglycan growth. The current study focused on charachterization of biosurfactant produced by L.plantarum and evaluated its antibacterial and antiadhesion activity
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