Abstract
Forty samples of different types of powdered infant milk had been collected including (celia 1-2 , biomil 1-2 , lery 1-2 , dialak 1-2 , albadee 2 ,nido 2 , kikos 2 , nictalia 1-2 , cerelak ). Some of the milk cans were opened directly at the beginning of test while others had been opened three days or more before testing. The results showed that Bacillus species were isolated and identified at different rates as Bacillus cereus has the highest rate (12.5%) then B.circulance and B. laterosporous at (5%) for each of them respectively, while B.megaterium was isolated at a rate of (2.5%). The ability of the isolated Bacillus species to produce enterotoxin was detected by the use of Rapid and delayed permeability test for the detection of the heat stable enterotoxin and the results were recorded by the appearance of blue non necrotic lesions after injection of the rabbit with the cell free culture supernated (CFCS) and Lysate supernatant (LS) of the isolated Bacillus species, and for the detection of heat labile enterotoxin, the paw oedema test was also used , the increase in thickness of fore and hind arms appeared. The ability of the Bacillus species to produce the enzyme lecithinase and lyse red blood cells were also studied and the results revealed that only Bacillus cereus produce the enzyme lecithinase. in addition all the isolates showed positive hemolytic activity.
Highlights
The results showed that Bacillus species were isolated and identified at different rates as Bacillus cereus has the highest rate (12.5%) B.circulance and B. laterosporous at (5%) for each of them respectively, while B.megaterium was isolated at a rate of (2.5%)
The ability of the Bacillus species to produce the enzyme lecithinase and lyse red blood cells were studied and the results revealed that only Bacillus cereus produce the enzyme lecithinase. in addition all the isolates showed positive hemolytic activity.
تؼ اتباع طخيقة ( )Haren at al 1990والسحؾرة عؽ طخيقة ( vartanyan et al )1977في اج اخء ىحا الاختبار .نلاحع في الذكل ( )3فأرة تؼ حقؽ اكفيا بػ 0.1سؼ 3مؽ مخؽ نقيع القمب والجماغ السعقؼ حيث سجل معجؿ سسػ الاكف الامامية والخمفية بعج 33ساعة مؽ الحقؽ ( )1ممؼ و ( )1.1ممؼ لكل مؽ الاكف الامامية والخمفية عمى التؾالي .وتؼ استخجاـ الفخنيا varnier caliperلقياس سسػ الاكف .وعشج مقارنتو بالأشكاؿ ( )4و ( )5التي تؾضح الدسػ في اكف الفئ اخف الامامية والخمفية السحقؾنة ب ()0.1سؼ 3مؽ اؿ ( )cell free culture supernated (CFCS)لبكتخيا الػ Bacillus السعدولة وصل سسكيا الى ( )2.9ممؼ للأكف الامامية في بعض السعاملات و ( (3.5ممؼ في الاكف الخمفية في معاملات اخخى (بعج 36ساعة مؽ الحقؽ).اما عشج حقؽ 0.1سؼ 3مؽ ( )Lysate supernatant (LS)لبكتخيا Bacillus.السعدولة فأعيخت الشتيجة اف سسكيا وصل اعمى ذروتو بعج 36ساعة حيث وصل الى 2.9ممؼ لكل مؽ الاكف الامامية و( )4ممؼ لكل مؽ الاكف الخمفية في
Summary
The results showed that Bacillus species were isolated and identified at different rates as Bacillus cereus has the highest rate (12.5%) B.circulance and B. laterosporous at (5%) for each of them respectively, while B.megaterium was isolated at a rate of (2.5%).
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