Study Regarding Age-Related Morphometric Features of Buffalo Sperm

Abstract

Semen production quality from reproduction males depends upon different factors such as:age, breed, collection method, environmental conditions. The present research was conducted to establish the influence of age upon the quantitative and qualitative indices of the native buffalo semen. There were analyzed 20 native buffalo males, from different age categories. At every semen collecting there were recorded the following morphologic and morphometric sperm indices: sperm volume, sperm concentration (x 106spz/ml), total number of sperm cells/ejaculate(x109), head length, head width, tail length, head area and shape factor.The influence of the age was made along two years, studying the dynamics of the sperm indices through the computer image analysis. Descripted statistics were performed on the recorded data to determine normality. Statistical analysis was performed as per standard statistical methods.The results revealed the fact that the best performances were recorded in bulls older than three years old. The study clearly demonstrates that there is a variation in reproductive parameters in the bovine bulls, which could be studied at the molecular level to unveil any genomic markers associated with low fertility and/or infertility. The males may be utilized to obtain semen by subjecting the young ones to training at an early age, thereby decreasing the initial age of semen donation.