Abstract
We studied the in vitro anti-tumor activity of Bidens Bipinnata L. extract. MTT assay was used to investigate the inhibitory effect of different concentrations of the extracts on human hepatocellular carcinoma (HepG2) cell lines and human cervical carcinoma (Hela) cell lines, and the IC50 values were calculated. The Bidens Bipinnata L. extract had different degrees of inhibitory effects on these two cells, and when exposure time was 48 h, the inhibition rate reached its peak, with IC50 values of 14.80 µg/mL and 13.50 µg/mL respectively. The Bidens Bipinnata L. extract had a good inhibitory effect on human HepG2 cell lines and Hela cell lines, and thus has certain development prospects.
Highlights
Bidens Bipinnata L. belongs to the genus Bidens, and it is widely used in the treatment of malaria, diarrhea, dysentery, hepatitis, acute nephritis and other diseases
The human HepG2 cell lines and human cervical carcinoma (Hela) cell lines were placed into the prepared DMEM medium or 1640 medium containing 10% fetal bovine serum, and cultured at 37°C in air containing 5% CO2, when the cells grew to about 80% of the sidewall, the old medium was discarded; the cells were digested with adequate amount of 0.25% trypsin, and the digestive fluid was discarded; culture medium was added, and the cells were passaged according to the proportion of 1:3, with one passage every three days, cells after recovery was regarded as the first generation cells, and the experiment was not be performed until the third generation cells were attained after the recovery
Human HepG2 cell lines and Hela cell lines in the logarithmic growth phase were collected respectively, and added with phosphate buffered saline (PBS) to flush out the debris, after trypsin digestion, cells were counted by blood cell counting chamber, and the cell density was adjusted to 5×104 cells/mL. 100 μl of the cell suspension was added into each well of the 96-well microplate, and PBS was added to the wells around the plate to maintain the humidity within the plate
Summary
Bidens Bipinnata L. belongs to the genus Bidens, and it is widely used in the treatment of malaria, diarrhea, dysentery, hepatitis, acute nephritis and other diseases. Pharmacological activities of the Bidens Bipinnata L. extract have been widely studied, which were mainly focused on the prevention of liver injury (Tang et al, 2006; Hu et al, 2007), anti-hyperlipidemia (Huang et al, 2009), analgesia (Zhou et al, 2007), treatment of renal anemia (Li et al, 2009), anti-hypertension (Qian et al, 2003), hypoglycemia (Li et al, 2003), anti-oxidative damage (Yang et al, 2006), and anti-tumor (Zhang et al, 2010). DMEM, 1640 medium (Beijing Borunlaite Science & Technology Co., Ltd.); fetal bovine serum (Beijing Biodee Biotechnology Co., Ltd.); trypsin (Jiangsu Yoke Chemical Co., Ltd.); MTT (Sigma); other reagents were domestically-made analytically pure ones
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
