Study on therapeutic efficacy of ultrasound destruction of VEGFI-carrying microbubbles in subcutaneous colon cancer xenograft in nude mice

Abstract

Objective To estimate the therapeutic efficacy of ultrasound destruction of vascular endothelial growth factor inhibitor (VEGFI)-carrying lipid microbubbles in colon cancer in nude mice.Methods Colon tumor xenografts cells were inoculated subcutaneously in eighteen Balb/c nude mice and tumor (5-20 mm) developed,all the mice were randomly divided into three groups:Group A (6 mice),as controls,underwent contrast-enhanced ultrasound (CEUS) examination and sham ultrasound radiation.Group B (6 mice) were given lipid microbubbles combined with ultrasound radiation.Group C (6mice)underwent VEGFI-carrying lipid microbubbles injection combined with ultrasound radiation.Contrastenhanced ultrasound (CEUS) examination was performed before and after ultrasound radiation.Enhanced peak intensity(PI),regional blood volume (RBV),regional blood flow (RBF) were recorded and compared.Results There was no significant difference in PI,RBV,RBF before radiation among the three groups (P＞0.05).PI,RBV,RBF in group A were higher 1 day after sham ultrasound radiation,the difference was not statistically significant (P ＞0.05),1 week later PI,RBV,RBF were higher than those before radiation,the difference was statistically significant (P ＜0.05).1 day after ultrasound radiation,PI,RBV,RBF in group B decreased,the difference was statistically significant (P ＜ 0.05),while after 1 week,PI,RBV,RBF increased respectively,comparing with those before ultrasound radiation,the difference was not statistically significant (P ＞0.05).The above indexes were significantly decreased in group C 1 day and 1 week after ultrasound radiation with statistically significant (P ＜ 0.05).Conclusions Ultrasound destruction of VEGFI-carrying lipid microbubbles can improve the therapeutic efficacy of colon cancer in nude mice. Key words: Ultrasonography; Microbubbles; Colonic neoplasms