Study on the therapeutic effect of high dose inosine to hypoxi-schemic brain damage in neonatal rats

Abstract

Objective To explore the therapeutic effect of high dose inosine to hypoxic-ischemic brain damage(HIBD)in neonatal rats; and provide some evidences for wide clinical usage. Methods 54 neonatal rats aged 1 week were randomly divided into sham, control and inosine group.HIBD models were made.The rats in the inosine group began injecting 0.1 % inosine(0.05 mg/g), twice a day; and the rats in the sham and control group saline solution with the same dose.The samples were made at the given time; and the levels of water, calcium and cell apoptosis in brain were investigated. Results ①The levels of wate at 3 d, 5 d in the control group, and at 5 d in the inosine group were higher than that in the sham group(P<0.05).As compared with the control group at the given time, the levels in the inosine group at 3 d and 5 d were all significantly decreased(P<0.05).② The contents of calcium in brain tissues peaked at 24 h, and then gradually decreased.The contents at 24 h, 3 d, 5 d in the control group and 24 h, 3 d in the inosine group were all significantly increased in comparison with the sham group(P<0.05).As compared with the control group at the given time, the contents at 24 h, 3 d, 5 d in the inosine group were all significantly decreased(P<0.05).③ The rates of brain cell apoptosis in the control and inosine group were all gradually increased(P<0.05).The rates in the control and inosine group at every given time were all significantly increased(P<0.05).The rates in the inosine group were all lower than that in the control group(P<0.05). Conclusion Inosine could remarkably protect cerebral tissue against HIBD. Key words: inosine; neonatal rat; hypoxic-ischemic brain damage; cell apoptosis