Abstract
Azole fungicides are widely used to control diseases and insect pests of vegetables, fruits and flowers because of their good antibacterial effect and regulating plant growth. However, their extensive use inevitably enters the water environment with rainfall and surface runoff, which leads to potential harm to aquatic organisms. At present, the research on the biological toxicity of azole fungicides mainly focuses on single azole compounds, such as Paclobutrazol, hexaconazole, propiconazole and Difenoconazole. However, the systematic study on the toxic effects of multi-component mixtures of azole fungicides is still very lacking, and it is unable to accurately assess the ecological risk. Therefore, in this paper, five common azole fungicides (imidazole, tebuconazole, triadimefon, tricyclazole and hymexazol) in the environment were taken as target pollutants. Scenedesmus obliquus was used as the toxicity indicator. The mixture system was designed by the equal effect concentration ratio method and linear average ray method. Study the 96h toxicity and interaction of Scenedesmus obliquus under combined stress of azole fungicides. The main research results are as follows:The 96 h toxicity test of five typical azole fungicides to Scenedesmus obliquus was determined by microplate toxicity analysis method. Fitting with Weibull function and taking EC50 as the criterion,the results show that the single toxicity order of the five tested fungicides was:tebuconazole > Triadimefon > oxacillin > Tricyclazole > imidazole.
Highlights
Fungicides usually refer to chemical preparations that can effectively control or kill microorganisms such as bacteria, fungi and algae in the water system environment [1], and generally in the international arena, can be used as agents for the control of various pathogenic microorganisms, known as as a fungicide
In terms of personal skin care products, azole fungicides are widely used in household products such as shampoos, skin care products, soaps, toothpastes and shower gels due to their low toxicity, broad spectrum and high efficiency [13], such as ketoconazole It is often used in anti-dandruff hair care formulations [14]
In order to reveal the ecotoxicity of azole fungicides exposed in the natural environment, this study took five azole fungicides as target pollutants, and investigated the effects of azole fungicides at different concentrations on the growth of the toxicity indicator organism, Scenedesmus obliquus
Summary
Fungicides usually refer to chemical preparations that can effectively control or kill microorganisms such as bacteria, fungi and algae in the water system environment [1], and generally in the international arena, can be used as agents for the control of various pathogenic microorganisms, known as as a fungicide. In 1944, Woolley successfully synthesized the first azole fungicide, benzimidazole During this period, after more than 70 years of development [5], azole fungicides have gradually become the protagonist of the sterilization market, and are widely used in agricultural production and personal skin care products. In order to reveal the ecotoxicity of azole fungicides exposed in the natural environment, this study took five azole fungicides as target pollutants, and investigated the effects of azole fungicides at different concentrations on the growth of the toxicity indicator organism, Scenedesmus obliquus. To clarify the single toxicity of these five typical azole fungicides to Scenedesmus obliquus, and to provide basic toxicity data for the risk assessment of mixed pollutants in the water environment
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