Abstract
Objective: The objective is to investigate whether acupuncture can protect brain by regulating the expression of ICAM-5 mRNA in brain tissue. Methods: Male SD rats were used to construct a middle cerebral artery occlusion reperfusion injury (Middle cerebral artery occlusion, MCAO) model, and the rats were randomly divided into 4 groups, namely the normal group (group A) and the sham operation group (group B), MCAO group (group C), MCAO + acupuncture group (group D); real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression of ICAM-5 mRNA in the brain tissue of the ischemic area, and HE staining and TUNEL were used to detect the level of neuronal apoptosis to evaluate the therapeutic effect. Results: Acupuncture can improve neuronal morphology and reduce neuronal apoptosis in rats with ischemic stroke. RT-qPCR: compared with group A and group B, the expression level of ICAM-5 mRNA in the brain tissues of group C and group D decreased, while compared with group C, The expression of ICAM-5 mRNA in the brain tissue of group D was significantly up-regulated (P 0.05). Conclusion: The effectiveness of acupuncture in the treatment of ischemic stroke may achieve neuroprotection by up-regulating the expression level of ICAM-5 mRNA.
Highlights
Stroke is the second leading cause of death in adults after cancer worldwide, and it is the main cause of adult disability
Male SD rats were used to construct a middle cerebral artery occlusion reperfusion injury (Middle cerebral artery occlusion, MCAO) model, and the rats were randomly divided into 4 groups, namely the normal group and the sham operation group, MCAO group, MCAO + acupuncture group; real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression of Intercellular adhesion molecule-5 (ICAM-5) mRNA in the brain tissue of the ischemic area, and HE staining and TUNEL were used to detect the level of neuronal apoptosis to evaluate the therapeutic effect
Zhou showed that the expression of ICAM-5 could be inhibited in the hypoxic-ischemic environment, and the expression of ICAM-5 protein has a neuroprotective effect in the hypoxic-ischemic environment, which can reduce the apoptosis caused by ischemia and hypoxia and promote cell survival [9]
Summary
Stroke is the second leading cause of death in adults after cancer worldwide, and it is the main cause of adult disability. Its occurrence is due to the disorder of brain blood supply and the decrease of cerebral blood flow below the critical level, resulting in insufficient delivery of oxygen and glucose, which triggers a series of pathophysiological events after acute stroke, mainly including energy failure, excitotoxicity, destruction of blood-brain barrier, inflammation and apoptosis, Eventually, it will lead to irreversible neuronal death [3] [4] [5]. Intercellular adhesion molecule-5 (ICAM-5) is a member of the immunoglobulin superfamily of adhesion molecules, which is expressed only on the surface of nerve cells. It plays an important role in promoting neuronal growth, nourishing nerves, and anti-neuronal apoptosis. Zhou showed that the expression of ICAM-5 could be inhibited in the hypoxic-ischemic environment, and the expression of ICAM-5 protein has a neuroprotective effect in the hypoxic-ischemic environment, which can reduce the apoptosis caused by ischemia and hypoxia and promote cell survival [9]
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