Abstract
Plasmids are extremely valuable as a source of DNA and for use in biotechnology. Our research goal was to develop plasmids with extremely high copy numbers for lab-scale plasmid preparations. Using the beverage method simplifies the process of obtaining a large quantity of plasmid DNA for various applications. Diet Coke as a beverage demonstrated that DNA recovery is highly adequate. Diet Sting with EcoRI and BamHI enzymes was used to detect restriction digestion with high sensitivity. However, for lab-scale preparative work, our findings show that plasmid yield can be significantly increased by using standard growth procedures and commonly used growth media.
Highlights
For more than a decade, geneticists have been interested in inducing mutations with chemicals and radiations with the goal of discovering mutagenic compounds through their specific activity, which can likely provide some understanding and knowledge of the chemical basis of mutation and gene structure [1]
In this gel plasmid and genomic DNA is seen affected by the different types of beverages
By doing this we observed that only Diet Coke and Sting were affected so Sting and Diet Coke was undergone restriction digestion for specific identity with EcoRI, BamHI and HindIII [Fig. 1A]
Summary
For more than a decade, geneticists have been interested in inducing mutations with chemicals and radiations with the goal of discovering mutagenic compounds through their specific activity, which can likely provide some understanding and knowledge of the chemical basis of mutation and gene structure [1]. Plasmids are extremely valuable as a source of DNA vaccines and in biotechnology applications. Plasmids have a significant impact on productivity, it is necessary to conduct research on plasmid stability and colony forming units. Plasmids can perform additional functions, such as plasmid-encoded genes that can improve the host's survival by completely killing a different organism or synthesizing toxins that act as defensive mechanisms. A single organism may contain multiple plasmids, each with a distinct function [6]. Restriction digestion is a technique that involves cutting a specific region of genetic material and incubating the targeted sequence with a restriction enzyme that binds to specific DNA sequences and cleaves at specific nucleotides [7]. Restriction digestion allows for the ligation of genetic material fragments, which is required for biological research [8]
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