Study on the pathological features of the bone marrow and JAK2 V617F gene mutation in patients with primary myelofibrosis

Abstract

Objective To investigate the pathological features of the bone marrow and the mutation of JAK2 V617F gene in patients with primary myelofibrosis (PMF), and analyze the effects of JAK2 V617F mutation on myelofibrosis and the number of hemocytes in patients with PMF. Methods The morphological features of 37 patients with PMF were studied by HGE and Gomori staining. The mutation of JAK2 V617F gene in marrow samples from patients was detected by fluorescence quantitative PCR. Results The positive rate of JAK2 V617F gene mutation in 37 patients with PMF was 54 % (20/37). JAK2 V617F positive patients had higher counts of white blood cell and platelet in peripheral blood [(12.79±3.54) × 109/L and (312.47±203.81) × 109/L] than JAK2 V617F negative patients [(4.22±2.07) × 109/L and (136.43±104.87) × 109/L] (t= 3.158, P= 0.025; t = 2.623, P= 0.046). The two groups had no significant difference between the counts of hemoglobin and the level of fiber hyperplasy in bone marrow(both P > 0.05). Conclusion JAK2 V617F gene mutation detection is important for diagnosis of PMF, especially those with high counts of white blood cell and platelet. Key words: Primary myelofibrosis; Pathology, clinic; JAK2 V617F gene mutation