Study on the number alteration of endothelial progenitor cells from peripheral blood in patients with acute myocardial infarction of young man

Abstract

Objective To compare the number of endothelial progenitor cells (EPCs) from peripheral blood in patients with acute myocardial infarction of young man and healthy man. Methods Eighteen young men (18~50 years old) with acute myocardial infarction (AMI) who were admitted at the First Affiliated Hospital of Dalian Medical University from June 2010 to April 2011 in young man were enrolled, aged (65~85 years old) men with acute myocardial infarction in 18 cases, within 24 hours of onset collected blood 2 ml. Ten cases of healthy young men (30~50 years old) were used as control group, fasting venous blood 2 ml. A volume (400 μl) of blood was taken to red blood cell lysis buffer hemolysis, labeled with vascular endothelial growth factor (VEGF), CD34, and CD133 antibodies, and then analyzed with flow cytometry. Results The number of EPCs in peripheral blood was measured in young male AMI group. The number of EPCs in peripheral blood was (0.58±0.83)% in older men. The number of EPCs in peripheral blood of AMI group was (0.04±0.03)%. For healthy controls, the number of EPCs was (0.02±0.02)%. The number of EPCs was significantly higher in AMI patients compared to control group (P<0.05). However, for AMI group, the increased number of EPCs in young men was significantly greater than young female (P<0.01). Conclusions The number of EPCs in peripheral blood in young man AMI patients is significantly increased within 24 hours. Key words: Myocardial infarction/BL; Endothelial cells/CY; Stem cells/CY