Abstract
Both Shuang-Huang-Lian and Xue-Bi-Jing injections are clinically proven efficient and low cost medicines. However, the studies of their chromatographic separation are limited. In this study, a column based on ionic liquid stationary phase was applied for the first time for the analysis of these two injections. For Shuang-Huang-Lian injection, U-shaped plots of retention factors vs. mobile phase constitutions were obtained. This unique kind of separation behavior allowed a flexible adjustment of retention by tuning the mobile phase constituents. Then acids group was analyzed with hydrophilic chromatography mode, while glycosides group was analyzed with reversed phase chromatography mode. Similarly, constituents in Xue-Bi-Jing injection were analyzed with mixed mode high performance liquid chromatography (HPLC). Higher, medium and lower polarity groups were analyzed with hydrophilic interaction, reversed phase and normal phase liquid chromatography modes, respectively. Results from this work provided valuable information for the separation of complicated systems such as traditional Chinese medicine injections.
Highlights
The formulations of traditional Chinese medicines (TCMs) are becoming increasingly popular worldwide, mainly because of their high efficiency and low cost
We used hydrochloric acid as the catalyst instead of triethylamine because we found that it was much easier to purify the ClPr‐Sil when acid was used as the catalyst
When triethylamine was used as catalyst, the elemental analysis results showed that even with extensively washing with water and ethanol, trace triethylamine was still absorbed on the silica
Summary
The formulations of traditional Chinese medicines (TCMs) are becoming increasingly popular worldwide, mainly because of their high efficiency and low cost. We prepared columns with methyl imidazole ionic liquid bonded silica as the stationary phase and applied them for the analysis of Xue-Bi-Jing (XBJ) and Shuang-Huang-Lian (SHL) injections. In this work we took the analysis of XBJ and SHL injections as the research objects of the mixed mode separation.
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