Study on the Mechanism of Salvia miltiorrhiza in the Treatment of Traumatic Bone Defects

Qian Tan,Weihua Ye,Ge Yang,Xin Hu,Yaoxi Liu,Ting Lei,Haibo Mei

doi:10.1155/2021/8646394

Abstract

Traumatic bone defect is one of the major orthopedic diseases in clinics, and its incidence is increasing year by year. And repairing traumatic bone defects is a very difficult problem in clinics at present. The surface of medical titanium-based alloy has good biological properties, and its implant has a certain role in promoting bone in bone tissue. However, titanium-based materials are biologically inert and have no biological activity. As a traditional Chinese medicine, Salvia miltiorrhiza has the efficacy of treating bone diseases and promoting bone healing. The curative effect can be better exerted by loading the traditional Chinese medicine active compound Salvia miltiorrhiza on the surface of the titanium implant in a certain way. At present, due to the complex chemical composition of Salvia miltiorrhiza, the mechanism of its use for the treatment of traumatic bone defects is still unclear. Therefore, in this study, we mainly discussed the potential target and mechanism of Salvia miltiorrhiza in the treatment of traumatic bone defects through network pharmacology, which may provide a scientific basis for the treatment of traumatic bone defects with Salvia miltiorrhiza loaded on the surface of medical titanium-based alloy. We screened out effective compounds and targets of Salvia miltiorrhiza and targets related to traumatic bone defects with the help of relevant databases. The targets of Salvia miltiorrhiza for traumatic bone defects were analyzed by STRING and GeneCards databases, and the results were visualized by constructing a compound-target network, protein-protein interaction network, and compound-target-disease network with Cytoscape 3.7.1 analysis software. Finally, the selected core targets carried out GO and KEGG enrichment. The results showed that 60 main active components were screened from Salvia miltiorrhiza Bunge, which could act on 149 targets. There were 33 active components and 70 targets related to traumatic bone defects, respectively. The core targets of Salvia miltiorrhiza in the treatment of traumatic bone defects were MAPK1, MAPK10, MAPK14, TGFB1, and TNF. The results of enrichment analysis showed that Salvia miltiorrhiza might treat traumatic bone defects through an osteogenic differentiation pathway.

Highlights

Bone is an organ that maintains the normal posture and movement of the human body and has the function of protecting internal organs [1]
Salvia miltiorrhiza has the effect of repairing bone defects, but its mechanism is unclear because of its complex composition. is study preliminarily studied the possible mechanism of Salvia miltiorrhiza in treating traumatic bone defects by using the network pharmacology method. e results showed that Salvia miltiorrhiza had the characteristics of multicomponent and multitarget in treating traumatic bone defects, and one component of Salvia miltiorrhiza can act on multiple targets
We found that osteoclast differentiation was an important signaling pathway through GO biological functions and KEGG pathway enrichment analysis, which corresponded to the above main components and targets. erefore, the active components luteolin, α-amyrin, cryptotanshinone, and tanshinone IIA in Radix Salviae Miltiorrhizae may play an important role in the treatment of traumatic bone defects, and its mechanism may be related to the regulation of MAPK1, MAPK10, MAPK14, TGFB1, TNF, JUN, ITGB3, CALCR, FOS, AKT1, PPARG, IFNG, RELA, and NFNB1 on the osteogenic differentiation pathway, so as to achieve the therapeutic effect

Summary

Introduction

Bone is an organ that maintains the normal posture and movement of the human body and has the function of protecting internal organs [1]. E repair and treatment of traumatic bone defects is a very difficult problem in clinical practice at present, and the treatment of bone defects is mainly through surgery and drug treatment. Bone defects must be filled and implanted with appropriate substances to accelerate bone repair [5]. Autograft is the preferred filler material due to its biocompatibility and bone induction potential, but its application is limited by the limited number of autologous bones and the surgical trauma it can cause at the donor site [6]. Erefore, at present, we mainly use synthetic bone materials instead of autograft for filling, so as to realize the complete integration Autograft is the preferred filler material due to its biocompatibility and bone induction potential, but its application is limited by the limited number of autologous bones and the surgical trauma it can cause at the donor site [6]. erefore, at present, we mainly use synthetic bone materials instead of autograft for filling, so as to realize the complete integration

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Conclusion