Study on the Mechanism of "Radix Pseudostellariae—Ligusticum Chuanxiong—Cassia Twig - Rehmannia" in Treating Chronic Heart Failure

Abstract

Objective: To explore the mechanism of "Radix pseudostellariae - Ligusticum Chuanxiong - cassia zhi - Rehmannia" in the treatment of cardiogenic heart failure through network pharmacology.Methods The drug targets of"Radix pseudostellariae - Ligusticum Chuanxiong - cassia zhi - Rehmannia" were screened based on TCMSP database and TCMID database, and the disease-related targets of heart failure were screened by GeneCard database. The intersection targets of active ingredients and disease-related targets were obtained, that is, the potential targets for drug treatment of chronic heart failure.The "drug-active ingredient-Potential target" network was constructed using Cytoscape 3.7.2 software, and the core active ingredients in the network were analyzed. The intersection targets were imported into STRING database for protein interaction (PPI) network analysis, and potential core targets were screened out. DAVID database was used to analyze the GO function and KEGG pathway enrichment of potential core targets.Results A total of 24 active ingredients and 193 potential targets were screened. The core active components such as luteolin, ursolic acid, sterol, stigmasterol and myriceone were obtained. STAT3, JUN, TP53, MAPK1, HSP90AA1, RELA, AKT1, ESR1, MAPK14, FOS, CCND1, IL6, RB1, CDKN1A, VEGFA, NR3C1, MAPK8, TNF, RXRA, AR core targets; A total of 173 signaling pathways were selected, including IL-17 signaling pathway, PI3K-Akt signaling pathway, TNF signaling pathway and calcium signaling pathway.Conclusion "Radix pseudostellariae - ligusticum Chuanxiong - cassia twidder-Rehmannia" plays a role in the treatment of chronic heart failure through the action of luteolin, ursolic acid, sterol, phytosterol, myriceones and other active components in IL-17 signaling pathway, PI3K-Akt signaling pathway, TNF signaling pathway, calcium signaling pathway and other pathways.