Study on the mechanism of qingre huoxue prescription in the intervention and treatment of acute myocardial infarction based on network pharmacology

Abstract

Objective: The objective is to study the mechanism of Qingre Huoxue prescription in the intervention and treatment of acute myocardial infarction (AMI) based on the method of network pharmacology. Materials and Methods: Five databases were used to screen the chemical compounds and targets of Ligusticum wallichii (chuanxiong), Radix Paeoniae Rubra (chishao), Lignum acronychiae (jiangxiang), Safflower (honghua), Salvia miltiorrhiza (danshen), Scutellaria baicalensis (huangqin), and Ilex pubescens (mao dong qing) in Qingre Huoxue prescription. Furthermore, Cytoscape-V3.2.1 software was used to construct the drug-component-target network. Functional protein association networks' database and the Database for Annotation, Visualization, and Integrated Discovery (DAVID) were used to visualize the protein interaction, pathway enrichment, and analysis. Results: A total of 44 active ingredients were screened out in Qingre Huoxue prescription. Among them, 178 targets and 41 compounds related to Qingre Huoxue prescription's function in treating AMI were obtained. After the analysis of the drug-component-action target network on Qingre Huoxue prescription, 14 key compounds and nine key targets with three scores above average were obtained. In addition, pathway enrichment and biological processes were conducted with the aid of the DAVID; and 8 related pathways and 10 biological processes were associated with AMI and related diseases; the PI3K-AKT signaling pathway, MAPK signaling pathway, and HIF-1 signaling pathway are the main pathways of Qingre Huoxue prescription for the treatment of AMI and related diseases. Conclusion: Qingre Huoxue prescription could treat AMI by multiple components, targets, and pathways. This study provides ideas and theoretical basis for further clinical studies on Qingre Huoxue prescription in treating AMI.