Study on the Mechanism of Periplaneta americana Extract to Accelerate Wound Healing after Diabetic Anal Fistula Operation Based on Network Pharmacology.

Fengfei Wang,Shuai Li,Juanni Zeng,Yuefei Geng,Le Ma,Yongmei Shen,Mohammad Hashem Hashempur

doi:10.1155/2021/6659154

Abstract

Objective Using network pharmacology research methods to explore the healing mechanism of American cockroach extract to accelerate wound healing after diabetic anal fistula surgery. Method The main chemical constituents of extracts from Periplaneta americana were collected by literature retrieval. Chemical composition and targets related to diabetic anal fistula wound could be predicted based on PubChem, Swiss Target Prediction, OMIM, and GeneCards databases, and the putative targets of Periplaneta americana extraction (PAE) for diabetic anal fistula wound were obtained by Venn diagram. These common targets were predicted using the String database for protein-protein interaction (PPI) network and then screening key genes through Cytohubba. Meanwhile, the above targets were analyzed using the DAVID database for gene ontology (GO) enrichment analyses and the Kyoto Encyclopedia of Genes and Genomes (KEGG) path enrichment analyses. Results A total of 12 chemical components of PAE were obtained by literature retrieval, and 61 therapeutic targets that may accelerate the healing of diabetic anal fistula wounds were predicted by the database. According to PPI network analysis, PAE accelerates wound healing after diabetic anal fistula surgery which may be related to proteins such as AKT1, VEGFA, EGFR, CASP3, STAT3, MAPK1, TNF, JUN, ESR1, and MMP9. GO analysis results show that targets of PAE to promote wound healing were mainly involved in biological processes such as cell proliferation, macrophage differentiation, angiogenesis, and response to hypoxia. KEGG analysis showed that the target genes were mainly concentrated in the PI3K-Akt signaling pathway, HIF-1 signaling pathway, and estrogen signaling pathway. Conclusion Periplaneta americana extract regulates multiple targets and multiple pathways to promote wound healing after diabetic anal fistula surgery. PI3K-Akt signaling pathway, HIF-1 signaling pathway, and sex hormone signaling pathway may be key pathways in the process of Periplaneta americana extract promoting wound healing.

Highlights

Anal fistula is a tube located between the perianal skin and the rectum
Periplaneta americana Treatment Targets for Diabetic Anal Fistula Wounds. 693 targets related to diabetic anal fistula wounds were retrieved through OMIM and GeneCards disease databases. 61 targets for the treatment of diabetic anal fistula wounds in Periplaneta americana were obtained by the Venn diagram online tool (Figure 1)
By using the Cytohubba analysis tool, the top 10 hub genes were obtained according to degree, and the results show that the hub genes are AKT1, VEGFA, EGFR, CASP3, STAT3, MAPK1, TNF, JUN, ESR1, and MMP9, as shown in Figure 3(b). ere is a strong interaction relationship, which may be a potential key gene for American cockroach extract to promote diabetic anal fistula wound repair

Summary

Introduction

Anal fistula is a tube located between the perianal skin and the rectum. It is caused by chronic infection and epithelialization of the drainage tube [1]. It is one of the common diseases in the anorectal department, and surgery is the first choice. Prolonged generation of pathological blood sugar elevation in the body can lead to the sequel of advanced glycation end products in the body, which provide a good nutritional environment for the growth of bacteria, and cause wound tissue infection and necrosis, and hinder wound healing. Diabetes is a risk factor leading to slow wound healing after anal fistula surgery [3,4,5]

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Discussion

Conclusion