Abstract
BackgroundHepatocellular carcinoma (HCC) is one of the most common malignant tumors with high mortality worldwide. Accumulating researches have indicated that long non‑coding RNAs (lncRNAs) are involved in varies human cancers, including HCC. Nevertheless, the specific molecular mechanism of lncRNA lysyl oxidase like 1 antisense RNA 1 (LOXL1-AS1) in HCC is still unclear.MethodsLOXL1-AS1 expression was tested via qRT-PCR in HCC cells. Functional and mechanism assays were respectively done to evaluate the biological functions of HCC cells and the potential interaction of LOXL1-AS1 and other factors.ResultsWe discovered that LOXL1-AS1 was high expressed in HCC cells. Inhibition of LOXL1-AS1 repressed cell proliferation, migration and invasion, but enhanced cell apoptosis in HCC. Further, miR-3614-5p was proven to be sponged by LOXL1-AS1. Additionally, Yin Yang 1 (YY1) was proven as the target gene of miR-3614-5p, and YY1 depletion could repress HCC cell malignant behaviors. YY1 could also transcriptionally activate LOXL1-AS1 expression. In rescue assays, we confirmed that overexpression of YY1 or miR-3614-5p inhibition could reverse the suppressive effects of LOXL1-AS1 silence on the malignant behaviors of HCC cells.ConclusionIn short, LOXL1-AS1/miR-3614-5p/YY1 forms a positive loop in modulating HCC cell malignant behaviors.
Highlights
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors with high mortal‐ ity worldwide
Data obtained from ENCORI reflected aberrantly higher expression of LOXL1-AS1 in liver hepatocellular carcinoma (LIHC) tissues compared with normal tissues (p = 0.0099, Additional file 1: Fig. S1A)
EdU assay further proved the repressive influences exerted by sh/LOXL1-AS1 on cell proliferative capability since we observed that EdU positive cells were significantly reduced in sh/LOXL1-AS1 transfection groups (Fig. 1D)
Summary
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors with high mortal‐ ity worldwide. Accumulating researches have indicated that long non‐coding RNAs (lncRNAs) are involved in varies human cancers, including HCC. The specific molecular mechanism of lncRNA lysyl oxidase like 1 anti‐ sense RNA 1 (LOXL1-AS1) in HCC is still unclear. Hepatocellular carcinoma (HCC) is one of the most common malignant tumors [1]. It has gradually become the second leading cause of cancer-related deaths in the whole world [2]. It is necessary to explore the molecular mechanism of HCC in depth and discover more effective targets for treatment, so as to improve the survival rate of patients
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
