Abstract
To the mechanism and therapeutic effect of Antelope horn treatment on cerebral ischemia reperfusion injuries under different levels of concentration. The process is as follows: (1) establish MCAO mouse model, test the modelling using traction and open field experiments, use TTC staining to detect the protective effect of Antelope horn treatment on cerebral ischemia reperfusion injury; (2) after 48 h of MCAO, use Nissl staining to measure the extent of hippocampal neuron damage in each group. The hippocampus corner 1 sector and dentate gyrus corner zone of hippocampal neurons of ischemia reperfusion group mouse suffered severe damages under Nissl staining detection. However, the hippocampal neurons of the medicated mouse show obvious alleviation. Western Blot results showed that, compared to the ischemic control group, the Bcl-2, Hsp 27, Hsp 27 expressions of the medicated group were significantly increased, while the Bax expression was dramatically reduced. Immunofluorescence outcome showed obvious reduction of TUNEL and FJB positive cells in the medicated group. That Antelope horn can reduce focal cerebral ischemia reperfusion injury. The reduction of degeneration and apoptosis suggests the correlation between the therapeutic effect of Antelope horn on ischemia reperfusion injury and its anti-apoptotic protective effect.
Highlights
Stoke, a series of diseases characterized by local neurological deficits due to brain blood circulation disorders, is one of the most serious threats to human health, ranking the no.1 disabling and no.2 killing disease (Sarkar et al, 2017; Chrzan et al, 2017)
Antelope horn is a traditional Chinese medicine with a good effect in removing heat and toxin, cooling blood detoxification, protecting liver, and improving eyesight. It is mainly used as an antispasmodic for detoxification swelling, heat shock epilepsy, coma spasm, eclampsia convulsions, epilepsy mad, headache, dizziness, red eyes, warm spot poison, and carbuncles sore etc.This study focuses on the application of Antelope horn in cerebral ischemia reperfusion injury model, where there may be a certain therapeutic effect
Antelope horn is a kind of traditional Chinese medicine of low toxin and high effectiveness, and our research had found its protective effect of cerebral ischemia reperfusion injury
Summary
A series of diseases characterized by local neurological deficits due to brain blood circulation disorders, is one of the most serious threats to human health, ranking the no. disabling and no. killing disease (Sarkar et al, 2017; Chrzan et al, 2017). Years of medical evidence has proved that the most effective treatment is intravenous thrombolysis at 3-4.5 h after onset. Certain modern medications are effective, but with relatively large side effects (Chen et al, 2016; Wang et al, 2016). Antelope horn is a traditional Chinese medicine with a good effect in removing heat and toxin, cooling blood detoxification, protecting liver, and improving eyesight. It is mainly used as an antispasmodic for detoxification swelling, heat shock epilepsy, coma spasm, eclampsia convulsions, epilepsy mad, headache, dizziness, red eyes, warm spot poison, and carbuncles sore etc.This study focuses on the application of Antelope horn in cerebral ischemia reperfusion injury model, where there may be a certain therapeutic effect
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