Abstract
A Gram positive bacterium strain 12-3 for degrading DDT effectively was isolated from the DDT contaminated site of the shipyard in Guangzhou by enrichment culture, which could utilize DDT as the sole carbon source for growth. This strain was identified as Pseudomonas species. Based on the phenotype, physiological and biochemical identification, and fatty acids identification. Testing theirs DDT degradation rate with HPLC, the results showed that in a shaky flask containing 20 mg/L DDT, this strain could degrade DDT with degradation efficiency of 51.6% in 8 days at 30°C, pH 8.0.
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