Abstract
The interaction between graphene oxide-sliver nanocomposites (GO-AgNCPs) and bovine serum albumin (BSA) in aqueous buffer solution was investigated by using several spectroscopic and imaging techniques. The visible absorbance intensity of GO-AgNCPs increased with increasing concentrations of BSA, and a slight redshift of the surface plasmon resonance band (SPR) occurred due to the absorption of BSA on the surface of GO-AgNCPs. Fluorescence data revealed a static quenching process of BSA caused by GO-AgNCPs. Thermodynamic parameters of the absorption process, including adsorption equilibrium constants, changes in Gibbs free energy (ΔG), enthalpy (ΔH) and entropy (ΔS), were evaluated at different temperatures. Negative values of ΔG showed that this process was spontaneous and the BSA-GO-AgNCPs complex might form in aqueous solution. Negative values of ΔH and ΔS suggested that the binding was mainly an enthalpy-driven process, and van der Waals forces and hydrogen bonding were the major force in the formation of the nanoparticle-protein corona. Analysis of synchronous, three dimensional (3D) fluorescence and circular dichroism (CD) spectra demonstrated that the conformation of BSA was slightly altered in the presence of GO-AgNCPs. The protein corona formed on the surface of GO-AgNCPs was directly observed by scanning probe microscopy (SPM).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Biological Macromolecules
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
