Abstract
The fluorescence enhancement of berberine hydrochloride (BBH) as a result of complex with β-cyclodextrin (β-CD) is investigated. The mechanism of the inclusion was studied and discussed by spectrofluoremetry and infrared spectrograms. The results showed that a 1∶1 (β-CD: BBH) complex was formed with an apparent association constant of 4.23×102 L/mol. Based on the enhancement of the fluorescent intensity of berberine hydrochloride, a new spectrofluorimetric method for the determination of BBH in the presence of β-CD was developed. The linear range was 1.00∼4.00 µg/mL with the detection limit of 5.54 ng/mL. The proposed method was successfully applied to the determination of BBH in tablets.
Highlights
Berberine hydrochloride (BBH,Fig. 1), an alkaloid originally isolated from Huanglian (Coptis chinensis), has been extensively used in China as a nonprescription drug to treat diarrhea caused by bacteria since 1950s with a confirmed safety
The analytical methods for the determination of BBH were HPLC [4,5], fluorescence quenching and enhancement methods [6,7,8], resonance Rayleigh scattering[9], visual colorimetric method[10] The spectrofluorimetric method has been widely used in the determination of biological samples environmental substances and pharmaceutical since it is highly sensitive, selective, operated and economic
Cyclodextrins (CDs, Fig.2) are cyclic oligosaccharides consisting of (a-1,4)-linked a-d-glucopyranose units, which have the property of forming inclusion complexes with guest molecules which possess suitable polarity and dimension
Summary
Berberine hydrochloride (BBH,Fig. 1), an alkaloid originally isolated from Huanglian (Coptis chinensis), has been extensively used in China as a nonprescription drug to treat diarrhea caused by bacteria since 1950s with a confirmed safety. Cyclodextrins (CDs, Fig.2) are cyclic oligosaccharides consisting of (a-1,4)-linked a-d-glucopyranose units, which have the property of forming inclusion complexes with guest molecules which possess suitable polarity and dimension. Having the ability to form inclusion complex by allowing other molecules into their hydrophobic cavity, CDs have been successfully used to improve solubility, chemical stability and bioavailability of a number of poorly soluble compounds. We found that an obvious increase of fluorescent intensity was observed when b-CD was added to the aqueous solution of BBH. The BBH/b-CD inclusion complex was prepared by freeze drying method and characterized by infra-red spectrograms. Based on the enhancement of the fluorescent intensity, BBH in aqueous solution was determined with high sensitivity and selectivity by spectrofluorimetry.
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