Abstract
AbstractA detection of anthracycline antitumor drug daunomycin (DNR) reacting with DNA in simulate metabolismin vitro has been made. It was found that DNR could react with DNA to form DNR‐DNA adducts. The adduct compositions of DNR with fish sperm DNA and thermally denaturated DNA were determined. The equilibrium association constant K of DNR with fish sperm DNA is 1.98×105 L/mol and that of DNR with denaturated DNA is 2.29×104 L/mol. Semiquinone free radicals, metabolic products of DNR, can destroy both fish sperm DNA and its thermally denaturated DNA. It is verified by hyperchromic effect increase observed in UV spectrum and AFM experiments. The mechanism of DNA degradation has also been investigated. Results obtained allow one to explain the reason of side effect of anthracycline drug and give the way to depress, which were of clinical significance.
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