Abstract
In the previous study, abnormal fluorescent substances which appear in the urine of patients of gastrointestinal diseases and their relationship with FAD were investigated by means of paper partition chromatography, and indoxyl sulfate was determined by absorptive phenomenon on Rf. 0.55, kynurenine showed bluish fluorescent spot on Rf. 0.45 under ultraviolet irradiation; and these two substances were observed to decrease after the administration of FAD. Therefore, relationship between FAD and metabolism of indole nucleus was experimentally investigated. Following results were obtained: 1) In the urine of normal rabbits, the excretion of metabolite of tryptophan system showed considerable fluctuation individually and daily. Dietary influences were, however, more marked: it was concluded that vitamins in the food, especially riboflavin, pyridoxine, folic acid and ascorbic acid showed much influences. 2) When tryptophan was given to rabbits, the urinary riboflavin showed increases two days after the cessation of the tryptophan administration. 3) Urinary excretion of indican in the patient with diseases of gastrointestinal system was confirmed to increase more than in normal person, and to decrease by the addition of FAD. 4) When indole was given orally to normal rabbits, excretion of urinary indican and urinary anthranilic acid increased. In case where FAD was given in addition, oxidation of indole to indican was acceralated and this indican was excreted more rapidly. The amount of indican was decreased by the addition of FAD. 5) When indole was given to the rat subcutaneously, indican content in the blood, liver, kidneys and lungs increased. If FAD was added to indole, indican content in them elevated in the early stage and then decreased more rapidly. In the other hand, tryptophan, kynurenine and anthranilic acid in the liver showed some tendency of increase after the administration of indole.
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