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Abstract
Using chitosan (CS) as carrier, the method named layer‐by‐layer (LBL) self‐assembly modification to modify the glassy carbon electrode (GCE) with multiwalled carbon nanotubes (MWNTs) and acetylcholine esterase (AChE) was proposed to prepare the acetylcholine esterase electrode with high sensitivity and stability. The modified electrode was used to detect pesticide of aldicarb, and the enzyme inhibition rate of the electrode showed good linearity with pesticide concentrations in the range of 10−10 g·L−1 to 10−3 g·L−1. The detection limit was 10−11 g·L−1. The modified electrode was also used to detect the actual sample, and the recovery rate range was from 97.72% to 107.15%, which could meet the rapid testing need of the aldicarb residue. After being stored in the phosphate buffer solution (PBS) in 4°C for 30 days, the modified electrode showed good stability with the response current that was 80% of the original current.
Highlights
At present carbamate pesticide residue detecting technology mainly includes such following types: enzyme inhibition based on biological detection techniques [1], immunoassay [2], chromatographic detection [3,4,5], and GC-MS&LC-MS [6]
In order to make more multiwalled carbon nanotubes (MWNTs) modified to the surface of electrodes, we dipped the probe into sodium borate solution of pH 9.18 for 15 min, carefully washed it with deionized water, and immersed it into 0.5% concentration of CS solution for 15 min
The electrode surface was rinsed with deionized water to get rid of redundant CS and immersed in the enzyme liquid of 100 U for 15 min and taken out and rinsed carefully with deionized water
Summary
At present carbamate pesticide residue detecting technology mainly includes such following types: enzyme inhibition based on biological detection techniques [1], immunoassay [2], chromatographic detection [3,4,5], and GC-MS&LC-MS [6]. The immobilized enzyme was firm, but violent reaction, poor mechanical performance, and low enzyme activity greatly affected the sensitivity and stability of obtained AChE. To improve the sensitivity and stability of obtained AChE, this paper adopted CS and MWNTs as modified materials to fix MWNTs and AChE by LBL technique and made highly efficient and stable AChE electrodes for the detection of pesticide aldicarb. It hardly destroys the enzyme molecule, and at the same time through multilayer modification it can prevent loss of enzyme and improve the activity of electrode. Biosensors based on AChE have become a promising technique for environmental monitoring, toxicity analysis, military investigations, and foodstuff quality [32, 33]
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