Abstract
Simultaneous saccharification and fermertation (SSF) were studied to produce ethanol from corn stover, which include three continuous stages, stover pretreatment, cellulose fermentation, and ethanol production. Firstly, the crude was treated by propionic acid, and the disposal rate of lignin and hemicellulose in corn stover was 60.6%, 98.5%, respectively, with a residual rate of cellulose 90.68%. Then, cellulase production was carried out byTrichoderma reeseiRut C-30. 10% stillage and other nutrition were added to the pretreated corn stover, water moisture and initial pH were adjusted to 70% and 4.8, liquid inoculum was (1.7~2.0)×107spore/g stover. The stover was kept under 30°C for 7d, then mixed with equal pretreated stover. Ratio of water was 2, the inoculum was 3.2×107cell (Saccharomyces cerevisiae)/g substrate, After incubated at 36°C for 72h, the SSF process achieved a yield of ethanol (0.326g/g substrate).
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