Abstract
This study investigates the optimisation of Black Soldier Fly oil extraction from different feeding sources. The larvae are fed a mixture of pasta-tobacco waste (MPT) and fish-ball sludge (FS). The mechanical press method is used to extract the oil. The results showed that the MPT-fed larvae had a higher weight at 0.088 ±0.001 g compared to 0.062 ± 0.002 g for larvae fed with FS. The feeding rates revealed that the higher the dose of the feed, the more weight the larvae gained. Meanwhile, GC-MS results show that the MPT substrate has a higher number of characterised fatty acids than the FS substrate, with three predominant fatty acids being lauric acid (43.69%), palmitic acid (16.15%) and linoleic acid (12.35%). Fourier-Transform Infrared spectroscopy (FTIR) analysis for both substrates reveals a similar high absorbance but High-Performance Liquid Chromatography (HPLC) analysis shows glutamic acid to be the most abundant amino acid in both substrates. Additionally, the regression analysis shows that moisture content and feeding rate is the most important parameter. Response Surface Methodology (RSM) determined that moisture content and feeding rates were the best parameters for oil yield.
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