Study on the Electrochemical Behaviour of Apigenin and Its Interaction with Dna

Abstract

The electrochemical behavior of the antitumor herbal drug apigenin was studied in 0.1 M B-R buffer solution (50% ethanol, pH 9.0) by cyclic voltammetry (CV), normal pulse voltammetry (NPV), chronoamperometry (CA) and chronocoulometry (CC) at glassy carbon electrode. In CV, only one irreversible anodic peak with Ep = 0.580V was appeared at scan rate of 50 mV/s and a new electroanalytical method for this herbal drug was established according to this anodic peak. The peak currents are linearly relationship with apigeninconcentrations in a range from 5.0×10-6 M to 9.0×10-5 M. Using the established method without pre-separation,apigenin in herbal drug was determined with satisfactory results. Moreover, the electrode process dynamics parameters were also investigated by electrochemical techniques and the possible electrode reaction mechanism was deduced. We also study the interaction of apigenin with DNA by DPV and Ultraviolet –Visible (UV) spectra. The results show apigenin doesn’t interact with DNA under the conditions.