Study on the effect of sodium-nanoparticle in rice root development

Abstract

ABSTRACT Micro-nanoparticles can enter the root tissue of plant cells along with the multiple lanes, and then accumulate in the tissue. But the plant physiological effect is still less studied. In this study, rice seedlings at germination stage were treated with 100 µM NaBiF4 and BiF3. We found that exogenous application of NaBiF4 treatment inhibited the elongation of rice roots and promoted the generation of adventitious roots, but treated BiF3 did not mediate obvious phenotype. Further analysis of the peroxidase activity in related tissues showed that NaBiF4 induced the activity of SOD and CAT decreased, and POD increased, while BiF3 only induced the activity of SOD to reduced, but the activity of CAT and POD were no changed. Further analysis of the sodium element and potassium element concentration in tissues showed that only the NaBiF4 treatment reduced content of potassium, but not sodium. Finally, stress-related genes OsMT1, OsMT2, OsOVP1, OsNIP2;1, and OsMT2b were analyzed by Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). These results showed that NaBiF4 induced the expression of OsMT2, OsOVP1, OsNIP2;1 decreased, and OsMT2b increased. However, BiF3 only induced expression of OsMT1 increased. These results provide a physiological basis for further analysis of the effects of sodium salt-nanoparticles in crop plants.