Abstract
Objective Experimental model of experimental autoimmune Myasthenia Gravis (EAMG) were established to explore the effect of Zini-Huangqi-Gegen decoction and Peiyuan-Guben powder on EAMG rats model. Methods Experimental animals were randomly divided into the control group (n=10) and the model group (n=30). The model rats were induced by murine AChR-α97-116 peptide immunostaining for EAMG rats model. After the first immunization, the general condictions and body weight of rats were observed, and the Lennon score was used to evaluate the rats. The second immunization was performed on the 11th day after the first immunization. On the 15th day after the first immunization, the rats were randomly divided into the model group(n=8), Huangqi-Gegen decoction and Peiyuan-Guben powder group (abbreviated Huangpei group, n=8) and Prednisone group (n=8) according to the Lennon score. Huangpi group rats were treated with Huangqi-Gegen decoction (21.5 g/kg) combined with Peiyuan-Guben powder (0.8 g/kg), prednisone group with 0.005 4 g/kg prednisone aqueous solution, the control group and the model group oral volume of distilled water. The rats were administered with a body weight of 10 ml/kg once a day for a total of 56 days. At the 70th day after the first immunization, serum was extracted from the rats. The Anti-AChR-α97-116 IgG and its subtype in serum were detected by ELISA. The IL-4, IL-10, IL-17 in serum were detected by ELISA. Results Compared with the model group, the weight of the rats in the Huangpei group and the prednisone group significantly increased after the 28th day of the first immunization (P<0.05). After the 36nd day of the first immunization, the Lennon score of the Huangpei group significantly decreased (P<0.05). At the end of the administration, the amplitude of EMG amplitude attenuation (41.83% ± 7.45% vs. 67.76% ± 4.32%) in the Huangpei group significantly decreased (P<0.05), and the serum IgG (1.15 ± 0.07 vs. 1.24 ± 0.08), IgG1 (0.17 ± 0.01 vs. 0.25 ± 0.03), IL-4 (16.54 ± 1.66 pg/ml vs. 25.64 ± 1.74 pg/ml), IL-10 ( 113.65 ± 12.87 pg/ml vs. 121.54 ± 10.44 pg/ml), IL-17 (43.58 ± 3.54 pg/ml vs. 65.76 ± 3.59 pg/ml) in the rat serum significantly decreased (P<0.05). Conclusions Huangqi-Gegen decoction and Peiyuan-Guben powder can increase the body weight of rats, decrease the concentration of AChR-Ab in serum, the concentrations of IL-4, IL-10 and IL-17 in serum, and effectively improve the symptoms of EAMG rats. Key words: Myasthenia gravis, autoimmune, experimental; Huangqi-Gegen decoction and Peiyuan-Guben powder; Inflammation; Rats
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