Abstract
Objective To study the effects of Huanglian Jiedu Decoction (HJD) on gut microflora of SD rats. Method After 3 days of adaptive feeding, 36 rats were randomly divided into 4 groups, namely, the normal control group (NC, 10 mL/kg, distilled water), high_HJD dose group (H_HJD, 6.25 g/kg, weight ratio between crude drug and rat), medium_HJD dose group (M_HJD, 3.125 g/kg), and low_HJD dose group (L_HJD, 1.56 g/kg), and each group consisted of 9 mice. The HJD groups were then treated with orally administered HJD for 21 days, while the NC group was treated with distilled water. After 7 days, 14 days, and 21 days of the experiment and after 12 hours of fasting and water deprivation, 3 SD rats in each group were randomly sacrificed by cervical dislocation and sterile operation to collect stool faces. Sample DNA was extracted by Fecal total DNA extraction kit, sequenced using Illumina MiSeq platform, and analyzed. Results The abundance of Romboutsia, Turicibacter, Lactobacillus, and Gemella decreased, while that of Escherichia_Shigella, Coprobacillus, Blautia, Akkermansia, Klebsiella, Rhodococcus, Parabacteroides, Citrobacter, Enterococcus, Bacteroides, and Erysipelotrichaceae_incertae_sedis increased after using H_HJD. The abundance of Gemella, Turicibacter, Romboutsia, and Lactobacillus decreased, while that of Blautia, Akkermansia, Escherichia_Shigella, Thiobacillus, Rothia, Enterococcus, Lactobacillus, and Erysipelotrichaceae-incertae-sedis increased after using M_HJD. The abundance of Romboutsia, Turicibacter, Lactobacillus, and Gemella decreased, while that of Escherichia_Shigella, Bacteroides, Akkermansia, Enterococcus, Rhodococcus, Parabacteroides, Desulfovibrio, Blautia, Fusobacterium, Rothia, and Streptococcus increased after using L_HJD. Conclusion HJD can regulate gut microbiota, and its effect varies with different dosage and duration of medication.
Highlights
As the normal microbiota in the intestine, gut microbiota plays an important role in the physiological functions of the intestine and plays an important role in the host’s physiology, metabolism, immunity, digestion, and nutrition uptake [1]
The composition of gut microbiota is altered in many disease states, including type 2 diabetes mellitus (T2DM), high blood pressure (BP), colitis, cardiovascular disease, and obesity [2]
We randomly divided the 36 rats into 4 groups, namely, the normal control group (NC, 10 mL/kg, distilled water), high_HJD dose group (H_HJD, 6.25 g/kg, weight ratio between crude drug and rat), medium_HJD dose group (M_HJD, 3.125 g/kg), low_HJD dose group (L_HJD, 1.56 g/kg), and each group consisted of 9 mice. e Huanglian Jiedu Decoction (HJD) groups were treated with orally administered HJD for 21 days, while the NC group was treated with distilled water
Summary
As the normal microbiota in the intestine, gut microbiota plays an important role in the physiological functions of the intestine and plays an important role in the host’s physiology, metabolism, immunity, digestion, and nutrition uptake [1]. The composition of gut microbiota is altered in many disease states, including type 2 diabetes mellitus (T2DM), high blood pressure (BP), colitis, cardiovascular disease, and obesity [2]. Dysbiosis in the gut microbiota of type 2 diabetes mellitus (T2DM) patients is characterized by a higher proportion of Bacteroides [3]. Li and his colleagues found that the gut microbiota in patients with hypertension had a higher percentage of an enterotype rich in bacteria from the genus Prevotella [4]. Huanglian Jiedu Decoction (HJD), first recorded in the book “Wai-Tai-MiYao” in the Tang dynasty, is a representative agent for clearing away heat and detoxification in traditional Chinese medicine [6].
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