Abstract
BACKGROUND: The activation and proliferation of T cells are based on the signal transduction after combinating of ligands and T cell antigen receptors(TCRs). As an adapter molecular, LAT(Linker for Activation of T Cells) is a kind of transmembrane protein which leads to a positive regulation for growth and differentiation of T cells[1,2]. Then, the palmitoylation of LAT is the foundation of signal transduction process. OBJECTIVE: By construct an tumor model with T leukemia Jurkat cells in nude mice, to research the effect of high expression of LAT to tumor load of mice. METHODS: Culturing the Jurkat cell line, the 5 weeks old female node mice were classified to three groups: high expression of LAT group, LAT mutation group and normal Jurkat group, each group had 8 mice. After pretreated with cyclophosphamide, 5×106/mice Jurkat cells were injected at armpit. Then observed the tumor of mice, measured weight of tumors, and HE stained of tumor tissues. Flow cytometry was used to detect proliferation of tumor cell in peripheral blood. ELISA was used to test the level of interleukia (IL)-2 in serum. RESULTS: After injected cells several days, the nude mice have appeared tumors under armpit and some symptoms like somnolent, anorectic and ect. The results of Flow cytometry and ELISA had shown that high expression of LAT group had the highest rate of tumor cells proliferation than other groups. CONCLUSION: It was successfully established the nude tumor model of T lymphocyte, confirmed the positive effect of LAT to the T cells activation and proliferation, provided a new target for acute T lymphocytic leukemia gene therapy.
Highlights
Balb/c nude mice (Balb/c-nude) is a new variety of mutant mouse formed in homozygosis of alleles of nude locus in VII linkage group
In Figure C, Jurkat cells transfected with LAT-M-EGFP, green fluorescence was in distribution, and couldn’t saw the gathering area(Figure 1)
It is important to the signal transduction process and reported that lipid rafts is a general term used to location on the membrane of plasma cells[12], LAT of describe microdomains at membrane, and enriched in non-palmitoylation is easy to be decomposed[13,14]
Summary
Balb/c nude mice (Balb/c-nude) is a new variety of mutant mouse formed in homozygosis of alleles of nude locus in VII linkage group It has no thymus and cannot generate normal T cells. It has been reported that they had established animal model in Balb/c nude mice by tumor tissue xenograft[3]. OBJECTIVE: By construct an tumor model with T leukemia Jurkat cells in nude mice, to research the effect of high expression of LAT to tumor load of mice. The results of Flow cytometry and ELISA had shown that high expression of LAT group had the highest rate of tumor cells proliferation than other groups. CONCLUSION: It was successfully established the nude tumor model of T lymphocyte, confirmed the positive effect of LAT to the T cells activation and proliferation, provided a new target for acute T lymphocytic leukemia gene therapy
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