Abstract

Biodegradation, a widely used treatment for petroleum-pollution seawater, except for its effective degradation, the degradation performance and restoration advantages are also worth studying. In this paper, petroleum-degrading bacteria (P1) with biosurfactant-producing bacteria (B2) were used to perform bioaugmentation. The degradation performance and restoration advantages during bioaugmentation were analyzed by UV spectrophotometry and high-throughput sequencing. The results indicated the degradation rate of diesel by bioaugmentation was 71.86 % in 45 days which exceed 38 % to that without bioaugmentation. In addition, the bacterial community of seawater altered after diesel exposure, especially the species of Pseudoalteromonas and Candidatus palagibacter. During bioaugmentation, Shannon index increased from 1.91 to 2.74, and bacterial community diversity of seawater recovered after a period of time.

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